Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Generation of conditional Stat2 KO mice. ( a ) Targeting strategy used to construct Stat2 fl/fl mice with exons 5 through 8 flanked with loxP sites. E represents exons with their corresponding number. Brown boxes depict exons in wild type allele and green boxes depict exons after successful integration of targeting vector. ( b ) Confirmation of loxP sites integration in the Stat2 gene by Southern blot analysis (9.8 kb DNA fragment). ( c ) Genotyping of mouse-tail DNA by PCR confirms deletion of Stat2 floxed allele after breeding with CMV-Cre mouse.

Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

Techniques: Construct, Plasmid Preparation, Southern Blot

Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Impaired IFN-I signaling after conditional deletion of Stat2 by CMV-Cre—mediated recombination. ( a ) Loss of Stat2 gene expression validated in four different organs by qRT-PCR analysis. ( b ) Western blot analyses performed on several tissues confirm global Stat2 deletion ( Stat2 Δ / Δ ) by ubiquitous CMV-Cre recombinase. ( c ) Splenocytes of the indicated genotypes were left untreated or treated with IFN-β for 6 h, and ISG expression was determined by qRT-PCR. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Data are presented as SEM of three independent experiments. n.s; not significant.

Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing

Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Stat2 Δ / Δ mice show enhanced tumor growth. ( a ) B16-F1 or ( b ) EL4 tumor cells were injected subcutaneously in WT, Stat2KO , Stat2fl/fl and Stat2 Δ/Δ . Values are shown as mean tumor volume determined over 20 days. Representative images of individual tumors are shown. *, p ≤ 0.05; **, p ≤ 0.01; and ***, p ≤ 0.001. n = 6–8 animals/group.

Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

Techniques: Injection

Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Stat2 Δ / Δ fibroblasts have impaired antiviral response to type I IFNs. ( a ) Lung fibroblasts derived from the indicated genotypes were pretreated with IFN-β for 24 h. Cells were then infected with VSV-GFP (green) at an MOI of 0.01 and imaged after 24 h. Representative bright field and fluorescent images are shown. ( b ) Western blot analysis shows STAT2 expression in fibroblasts of indicated genotypes. Representative images are shown of n = 2.

Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

Techniques: Derivative Assay, Infection, Western Blot, Expressing

Journal: Immuno

Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

doi: 10.3390/immuno6010007

Figure Lengend Snippet: Efficient targeted deletion of Stat2 in mouse tissues. Expression of STAT2 and STAT1 was analyzed in various tissues by Western blot analysis. ( a ) Bone marrow–derived cDCs generated from Stat2 fl/fl mice crossed with CD11c-Cre mice ( Stat2 Δ-cDC ). ( b ) Colons from Stat2 fl/fl mice crossed with Cdx2-Cre mice ( Stat2 Δ-CE ). ( c ) Lungs from Stat2 Δ-cDC and Stat2 Δ-CE mice were included for specificity of targeted deletion. Wild-type (WT) and Stat2KO mice served as positive and negative controls, respectively. ACTIN was used as an internal protein loading control.

Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

Techniques: Expressing, Western Blot, Derivative Assay, Generated, Control